研究キーワード
酵母, ストレス応答, 小胞体
研究分野
ライフサイエンス / 細胞生物学 / 細胞生物学, ライフサイエンス / 応用生物化学 / 酵母遺伝学
研究室概要
微生物が進化の過程で獲得した様々な「環境ストレス」に対する適応機構について、分子・代謝・細胞レベルで解明し、多様な微生物機能を理解するとともに、微生物育種・物質生産などの技術開発を通して、バイオテクノロジーへの貢献を目指した研究・教育を行う。
学術論文
"Induction of Endoplasmic Reticulum Stress by Prodigiosin in Yeast Saccharomyces cerevisiae," Current Issues in Molecular Biology, vol.46, no.3, pp1768-1776, 26 Feb. 2024
Sy Le Thanh Nguyen, Thi Hien Trang Nguyen, Thi Tuyen Do, Thi Thao Nguyen, Thanh Hoang Le, Thi Anh Tuyet Nguyen, Yukio Kimata
[ NAISTレポジトリ ] [ doi:10.3390/cimb46030116 ]
"Enforcement and Enlargement of the Saccharomyces cerevisiae Endoplasmic Reticulum through Artificial Evocation of the Unfolded Protein Response," IGMin Research, vol.2, no.1, pp36-38, 30 Jan. 2024
Masaki Monguchi, Yukio Kimata
[ NAISTレポジトリ ] [ doi:10.61927/igmin142 ]
"Signal sequence-triage is activated by translocon obstruction sensed by an ER stress sensor IRE1α," Cell Structure and Function, 3 Nov. 2023
Ashuei Sogawa, Ryota Komori, Kota Yanagitani, Miku Ohfurudono, Akio Tsuru, Koji Kadoi, Yukio Kimata, Hiderou Yoshida, Kenji Kohno
[ doi:10.1247/csf.23072 ]
"Fundamental and Applicative Aspects of the Unfolded Protein Response in Yeasts," Journal of Fungi, Oct. 2023
Yuki Ishiwata-Kimata, Yukio Kimata
[ doi:10.3390/jof9100989 ]
"Endoplasmic stress sensor Ire1 is involved in cytosolic/nuclear protein quality control in Pichia pastoris cells independent of HAC1," Frontiers in microbiology, vol.14, pp1157146, 20 Jun. 2023
Yasmin Nabilah Binti Mohd Fauzee, Yuki Yoshida, Yukio Kimata
[ doi:10.3389/fmicb.2023.1157146 ]
"An ER-accumulated mutant of yeast Pma1 causes membrane-related stress to induce the unfolded protein response," Biochem Biophys Res Commun ., vol.667, pp58-63, May. 2023
Huong Thi Phuong, Yuki Ishiwata-Kimata, 木俣 行雄
[ NAISTレポジトリ ] [ doi:10.1016/j.bbrc.2023.05.044 ]
"Fast-growing Saccharomyces cerevisiae cells with constitutive unfolded protein response and their potential for lipidic molecule production," Applied and Environmental Microbiology, 30 Sep. 2022
Phuong Thi Mai Nguyen, Yuki Ishiwata-Kimata, Yukio Kimata
[ NAISTレポジトリ ]
"Self-association status-dependent inactivation of the endoplasmic reticulum stress sensor Ire1 by C-terminal tagging with artificial peptides," Bioscience, Biotechnology, and Biochemistry, vol.86, no.6, pp739-746, 24 May. 2022
Yuki Ishiwata-Kimata, Tatsuya Hata, Yukio Kimata
[ NAISTレポジトリ ] [ doi:10.1093/bbb/zbac038 ]
"Induction of the Unfolded Protein Response at High Temperature in Saccharomyces cerevisiae," International Journal of Molecular Sciences, vol.23, no.3, pp1669, 31 Jan. 2022
Tatsuya Hata, Yuki Ishiwata-Kimata, Yukio Kimata
[ doi:10.3390/ijms23031669 ]
"Induction and Aggravation of the Endoplasmic-Reticulum Stress by Membrane-Lipid Metabolic Intermediate Phosphatidyl-N-Monomethylethanolamine," Frontiers in Cell and Developmental Biology, vol.9, pp743018, 6 Jan. 2022
Yuki Ishiwata-Kimata, Quynh Giang Le, Yukio Kimata
[ doi:10.3389/fcell.2021.743018 ]
"Aeration mitigates endoplasmic reticulum stress in Saccharomyces cerevisiae even without mitochondrial respiration," Microbial Cell, vol.8, pp77-86, Jul. 2021
Huong Thi Phuong, Yuki Ishiwata-Kimata, Yuki Nishi, Norie Oguchi, Hiroshi Takagi, Yukio Kimata
[ NAISTレポジトリ ]
"Multiple Ways for Stress Sensing and Regulation of the Endoplasmic Reticulum-stress Sensors," Cell Structure and Function, vol.46, no.1, pp37-49, 22 May. 2021
Quynh Giang Le
"The ADP-binding kinase region of Ire1 directly contributes to its responsiveness to endoplasmic reticulum stress," Scientific Reports, vol.11, pp4506, 24 Feb. 2021
Quynh Giang Le, Yuki Ishiwata-Kimata, Thi Huong Phuong, Shigeto Fukunaka, Kenji Kohno
[ doi:10.1038/s41598-021-83890-x ]
"The unfolded protein response in Pichia pastoris without external stressing stimuli," FEMS yeast Research, vol.20, no.6, ppfoaa053, 14 Sep. 2020
Yasmin Nabilah Binti Mohd Fauzee, Naoki Taniguchi, Yuki Ishiwata-Kimata, Hiroshi Takagi, Yukio Kimata
[ doi:10.1093/femsyr/foaa053 ]
"出芽酵母オルガネラの危機管理 〜小胞体ストレス応答研究の最近の潮流〜," 化学と生物, vol.58, no.7, pp404-410, 2020.7.1
木俣 有紀, 木俣 行雄
"The unfolded protein response alongside the diauxic shift of yeast cells and its involvement in mitochondria enlargement," Scientific Reports, vol.9, pp12780, Aug. 2019
Duc Minh Tran, Yuki Ishiwata-Kimata, Thanh Chi Mai, Minoru Kubo
[ NAISTレポジトリ ] [ doi:10.1038/s41598-019-49146-5 ]
"Categorization of endoplasmic reticulum stress as accumulation of unfolded proteins or membrane lipid aberrancy using yeast Ire1 mutants.," Biosci. Biotech. Biochem, vol.83, no.2, pp326-329, Feb. 2019
D. M. Tran, H. Takagi, Y. Kimata
[ NAISTレポジトリ ] [ doi:10.1080/09168451.2018.1530098 ]
"Monitoring ADP/ATP ratio in yeast cells using the fluorescent-protein reporter PercevalHR," Biosci Biotechnol Biochem., vol.83, no.5, pp824-828, 31 Jan. 2019
Nguyen PTM, Ishiwata-Kimata Y
[ doi:10.1080/09168451.2019.1574204 ]
"Stress-sensing and regulatory mechanism of the endoplasmic-stress sensors Ire1 and PERK," Endoplasmic Reticulum stress in Diseases, vol.5, no.1, pp1-10, 26 Oct. 2018
Yuki Ishiwata-Kimata, Giang Quynh Giang, Yukio Kimata
[ NAISTレポジトリ ] [ doi:10.1515/ersc-2018-0001 ]
"A chimeric mutant analysis in yeast cells suggests BiP independent regulation of the mammalian endoplasmic reticulum-stress sensor IRE1α," Biosci Biotechnol Biochem., vol.82, no.9, pp1527-1530, Sep. 2018
Mai TC, Munakata T, Tran DM, Hiroshi Takagi
[ NAISTレポジトリ ] [ doi:10.1080/09168451.2018.1478716 ]
"The unfolded protein response of yeast Saccharomyces cerevisiae and other organisms," Plant Morphology, vol.30, pp15-24, Apr. 2018
Duc MInh Tran, Yukio Kimata
"4-Phenylbutyrate suppresses the unfolded protein response without restoring protein folding in Saccharomyces cerevisiae," FEMS Yeast Research, vol.18, no.2, ppfoy016, Mar. 2018
Thanh Chi Mai, Giang Quynh Le, Yuki Ishiwata-Kimata, 高木 博史, 河野 憲二, 木俣 行雄
[ NAISTレポジトリ ] [ doi:10.1093/femsyr/foy016 ]
"4-Phenylbutyrate suppresses the unfolded protein response without restoring protein folding in Saccharomyces cerevisiae.," FEMS Yeast Research, 14 Feb. 2018
Chi Thanh Mai, Quynh Giang Le, Yuki Ishiwata-Kimata, 高木 博史, 河野 憲二, 木俣 行雄
"Cadmium impairs protein folding in the endoplasmic reticulum and induces the unfolded protein response," FEMS Yeast Research , vol.16, no.5, 12 Jun. 2016
Le, Q.G,, Ishiwata-Kimata, Y., Kenji Kohno, Yukio Kimata
[ doi:10.1093 ]
"Retention of chimeric Tat2-Gap1 permease in the endoplasmic reticulum induces unfolded protein response in Saccharomyces cerevisiae.," FEMS Yeast Res., vol.15, no.5, Aug. 2015
Mochizuki T, Kimata Y, Uemura S, Abe F
[ doi:10.1093/femsyr/fov044 ]
"Tight regulation of the unfolded 1 protein sensor Ire1 by its intramolecularly antagonizing subdomain," Journal of Cell Science, vol.128, no.9, pp1762-1772, 1 May. 2015
Mathuranyanon, R, Tsukamoto, T, Takeuchi, A, Ishiwata-Kimata, Y, Tuchiya, Y, Kohno, K, Kimata, Y.
[ NAISTレポジトリ ] [ doi:10.1242/jcs.164111 ]
"Ethanol stress impairs protein folding in the endoplasmic reticulum and activates Ire1 in Saccharomyces cerevisiae.," Biosci. Biotechnol. Biochem, vol.78, no.8, pp1389-1391, 12 Jun. 2014
Miyagawa K, Ishiwata-Kimata Y., Kenji Kohno, Yukio Kimata
[ NAISTレポジトリ ] [ doi:10.1080/09168451.2014.921561 ]
"F-actin and a type-II myosin are required for efficient clustering of the ER stress sensor Ire1.," Cell Struct. Funct., vol.38, no.2, pp135-143, 6 Jul. 2013
Ishiwata-Kimata, Y., Yamamoto, Y.H., Takizawa, K., Hiraoka, Y., Kenji Kohno, Yukio Kimata
"Zinc depletion activates the endoplasic reticulum-stress sensor Ire1 via pleiotropic mechanisms.," Biosci. Biotechnol. Biochem. , vol.77, no.6, pp1337-1339, 7 Jun. 2013
Nguyen, L.S.T., Kenji Kohno, Yukio Kimata
"A BiP-bound and non-clustered mode of Ire1 evokes a weak but sustained unfolded protein response. ," Genes Cells, vol.18, no.4, pp288-301, Apr. 2013
Ishiwata-Kimata, Y., Promlek, T., Kenji Kohno, Yukio Kimata
[ doi:10.1111/gtc.12035 ]
"化学同人「酵母」," 第8章 タンパク質の変性・凝集と品質管理, vol.印刷中, 2013.3
木俣行雄
"Membrane aberrancy and unfolded proteins activate the endoplasmic reticulum-stress sensor Ire1 by different manners.," Mol. Biol. Cell,, vol.22, no.18, pp3520, Jun. 2012
Promlek, T, Ishiwata-Kimata, Y, Shido, M, Sakuramoto, M, Kohno, K., Kimata, Y.
"Translational pausing ensures membrane-targeting and cytoplasmic splicing of XBP1u mRNA.," Science, vol.331, pp586, Jan. 2011
Yanagitani, K, Kimata, Y, Kadokura, H. Kohno, K.
[ doi:10.1126/science.1197142 ]
"A novel ER J-protein DNAJB12 accelerates ER-associated degradation of membrane proteins including CFTR.," Cell Structure and Function, vol.35, pp107, Dec. 2010
Yamamoto, Y, Kimura, T, Momohara, S, Takeuchi, M, Tani, T, Kimata, Y, Kadokura, H, Kohno, K.
"ER stress-sensing mechanisms in yeast and mammalian cells.," Current Opinion in Cell Biology, ppPMID: 21093243, Nov. 2010
Kimata, Y, Kohno, K.
[ doi:10.1016/j.ceb.2010.10.008 ]
"Cotranslational targeting of XBP1 protein to the membrane promotes cytoplasmic splicing of its own mRNA.," Mol. Cell, vol.34, pp191, Apr. 2009
Yanagitani, K, Imagawa, Y, Iwawaki, T, Hosoda, A, Saito, M, Kimata, Y, Kohno, K.
[ doi:10.1016/j.molcel.2009.02.033 ]
"Saccharomyces cerevisiae Rot1 is an essential molecular chaperone in the endoplasmic reticulum.," Mol. Biol. Cell, vol.19, no.8, pp3514, Aug. 2008
Masato Takeuchi, Yukio Kimata, Kenji Kohno
[ doi:10.1091/mbc.E07-12-1289 ]
"Saccharomyces cerevisiae Rot1 is an essential molecular chaperone in the endoplasmic reticulum.," Mol. Biol. Cell, vol.19, no.8, pp3514, Aug. 2008
Masato Takeuchi, Yukio Kimata, Kenji Kohno
[ doi:10.1091/mbc.E07-12-1289 ]
"Two regulatory steps of ER-stress sensor Ire1 involving its cluster formation and interaction with unfolded proteins.," J Cell Biol, vol.179, pp75, Oct. 2007
Kimata, Y, Ishiwata-Kimata, Y, Ito, T, Hirata, A, Suzuki, T, Oikawa, D, Takeuchi, M, Kohno, K.
[ doi:10.1083/jcb.200704166 ]
"Two regulatory steps of ER-stress sensor Ire1 involving its cluster formation and interaction with unfolded proteins.," J Cell Biol, vol.179, pp75, Oct. 2007
Kimata, Y, Ishiwata-Kimata, Y, Ito, T, Hirata, A, Suzuki, T, Oikawa, D, Takeuchi, M, Kohno, K.
[ doi:10.1083/jcb.200704166 ]
"Transgenic mice expressing a fully nontoxic diphtheria toxin mutant, not CRM197 mutant, acquire immune tolerance against diphtheria toxin.," J Biochem (Tokyo), vol.142, pp105, Jul. 2007
Kimura, Y, Saito, M, Kimata, Y., Kohno, K.
"Transgenic mice expressing a fully nontoxic diphtheria toxin mutant, not CRM197 mutant, acquire immune tolerance against diphtheria toxin.," J Biochem (Tokyo), vol.142, pp105, Jul. 2007
Kimura, Y, Saito, M, Kimata, Y., Kohno, K.
"Self-association and BiP dissociation are not sufficient for activation of the ER stress sensor Ire1.," J Cell Sci, vol.120, no.9, pp1681, May. 2007
Oikawa, D, Kimata, Y., Kohno, K.
"Self-association and BiP dissociation are not sufficient for activation of the ER stress sensor Ire1.," J Cell Sci, vol.120, no.9, pp1681, May. 2007
Oikawa, D, Kimata, Y., Kohno, K.
受賞
"Best poster award," 14th International Congress on Yeasts, Sep. 2016
Ayano Kunimori, Yuki Ishiwata-Kimata, Yukio Kimata
所属学協会
酵母遺伝学フォーラム
日本農芸化学会
日本細胞生物学会
資料

渡辺 大輔

職位 准教授
領域 バイオサイエンス領域 統合システム生物学分野
研究室 ストレス微生物科学

微生物が進化の過程で獲得した様々な「環境ストレス」に対する適応機構について、分子・代謝・細胞レベルで解明し、多様な微生物機能を理解するとともに、微生物育種・物質生産などの技術開発を通して、バイオテクノロジーへの貢献を目指した研究・教育を行う。

両角 佑一

職位 助教
領域 バイオサイエンス領域 統合システム生物学分野
研究室 ストレス微生物科学

微生物が進化の過程で獲得した様々な「環境ストレス」に対する適応機構について、分子・代謝・細胞レベルで解明し、多様な微生物機能を理解するとともに、微生物育種・物質生産などの技術開発を通して、バイオテクノロジーへの貢献を目指した研究・教育を行う。

中瀬 由起子

職位 助教
領域 バイオサイエンス領域 統合システム生物学分野
研究室 ストレス微生物科学

微生物が進化の過程で獲得した様々な「環境ストレス」に対する適応機構について、分子・代謝・細胞レベルで解明し、多様な微生物機能を理解するとともに、微生物育種・物質生産などの技術開発を通して、バイオテクノロジーへの貢献を目指した研究・教育を行う。

磯貝 章太

職位 特任助教
領域 バイオサイエンス領域 統合システム生物学分野
研究室 ストレス微生物科学

微生物が進化の過程で獲得した様々な「環境ストレス」に対する適応機構について、分子・代謝・細胞レベルで解明し、多様な微生物機能を理解するとともに、微生物育種・物質生産などの技術開発を通して、バイオテクノロジーへの貢献を目指した研究・教育を行う。

木俣 有紀

職位 博士研究員
領域 バイオサイエンス領域 統合システム生物学分野
研究室 ストレス微生物科学

微生物が進化の過程で獲得した様々な「環境ストレス」に対する適応機構について、分子・代謝・細胞レベルで解明し、多様な微生物機能を理解するとともに、微生物育種・物質生産などの技術開発を通して、バイオテクノロジーへの貢献を目指した研究・教育を行う。

棚橋 亮弥

職位 博士研究員
領域 バイオサイエンス領域 統合システム生物学分野
研究室 ストレス微生物科学

微生物が進化の過程で獲得した様々な「環境ストレス」に対する適応機構について、分子・代謝・細胞レベルで解明し、多様な微生物機能を理解するとともに、微生物育種・物質生産などの技術開発を通して、バイオテクノロジーへの貢献を目指した研究・教育を行う。